Original Article
Association between plasma membrane Ca2+-ATPase gene polymorphisms and noise-induced hearing loss in a Chinese population
Abstract
Background: Noise-induced hearing loss (NIHL) has become a world-wide high occupational health risk. The current research is aimed at investigating the association of plasma membrane Ca2+-ATPase isoform 2 (PMCA2) gene polymorphisms with the susceptibility to NIHL in a Chinese population.
Methods: A total of 2,344 workers exposed to occupational noise were examined with hearing tests. According to the results of audiometry, we selected 613 cases with high frequency hearing threshold worse than 25 dB and 615 controls with high frequency hearing threshold better than or equal to 25 dB and the controls were frequency matched with cases in terms of age, gender and noise exposure level and exposure time. The individual genotypes for PMCA2 single nucleotide polymorphisms (SNPs) were determined using a TaqMan MGB probe assay performed on an ABI PRISM® 7900 HT Fast Real-Time PCR System (Applied Biosystems). Information about these subjects was collected by questionnaires which were conducted through face-to-face interviews by trained interviewers.
Results: We found that individuals with the rs3209637 CC genotype had a statistically significantly increased risk of NIHL compared with those who carried the rs3209637 TT genotype (adjusted OR =1.26, 95% CI =1.07–1.48) and this increased risk was more pronounced among the workers in the 15- to 25-year noise exposure time, >92 dB(A) noise exposure level. Similar effects were also observed in a recessive model.
Conclusions: Our data suggested that the PMCA2 polymorphisms may be a genetic susceptibility marker for NIHL in the Chinese Han population.
Methods: A total of 2,344 workers exposed to occupational noise were examined with hearing tests. According to the results of audiometry, we selected 613 cases with high frequency hearing threshold worse than 25 dB and 615 controls with high frequency hearing threshold better than or equal to 25 dB and the controls were frequency matched with cases in terms of age, gender and noise exposure level and exposure time. The individual genotypes for PMCA2 single nucleotide polymorphisms (SNPs) were determined using a TaqMan MGB probe assay performed on an ABI PRISM® 7900 HT Fast Real-Time PCR System (Applied Biosystems). Information about these subjects was collected by questionnaires which were conducted through face-to-face interviews by trained interviewers.
Results: We found that individuals with the rs3209637 CC genotype had a statistically significantly increased risk of NIHL compared with those who carried the rs3209637 TT genotype (adjusted OR =1.26, 95% CI =1.07–1.48) and this increased risk was more pronounced among the workers in the 15- to 25-year noise exposure time, >92 dB(A) noise exposure level. Similar effects were also observed in a recessive model.
Conclusions: Our data suggested that the PMCA2 polymorphisms may be a genetic susceptibility marker for NIHL in the Chinese Han population.
